Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: Expression of ChREBP, GLUT2 and GLUT1 in different clinical stages

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Expressing

Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: Histograms of protein expression in different liver tissues. Histograms of scores of ChREBP ( a ), GLUT2 ( b ), GLUT1( c ) expression levels in different stages of liver cancer. 0, absent of positive signal; 1, mild staining; 2, middle staining; 3, strong staining; 4, very strong staining. One-way ANOVA was used to analyze the correlation between protein expression and liver malignancy. Student-T test or Mann-Whitney U test was used to analyze the protein expression differences between 2 different clinical stages. **, P < 0.01; ***, P < 0.001

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Expressing, Staining, MANN-WHITNEY

Journal: Pathology Oncology Research

Article Title: Expressions of Carbohydrate Response Element Binding Protein and Glucose Transporters in Liver Cancer and Clinical Significance

doi: 10.1007/s12253-019-00708-y

Figure Lengend Snippet: GLUT1 but not GLUT2 protein co-expressed in ChREBP-positive hepatocytes in HCC . Immunohistochemistry of ChREBP-positive malignant hepatocytes ( a ) showing GLUT2 negative ( c ) but GLUT1 positive ( e ) staining. Arrow heads indicating the same cell. Immunohistochemistry of ChREBP-negative malignant hepatocytes ( b ) showing GLUT2 positive on the membrane ( d ) but GLUT1 negative staining ( f ). Arrow indicating the same cell. Scale bar = 30 μm

Article Snippet: The primary antibody for GLUT2 (Novus Biologicals, NBP2-22218SS, USA), GLUT1 (Abcam, ab115730, USA), were used at a 1:500 dilution and anti-ChREBP (Novus Biologicals, NB400–135, USA) was used at a 1:200 dilution.

Techniques: Immunohistochemistry, Staining, Membrane, Negative Staining